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Name : Smita Singh
Title : Biotechnician
EmailID : samitasingh@yahoo.com
Phone # : 3217459428
City : Melbourne
State : Florida
Country : USA
Skills :
Date : 2009-02-27
Experience :
Details
RESUME
Smita Singh
151, Eber Road, Apt#406, Melbourne, Fl-32901
Phone: (321)-745-9428
Email: samitasingh@yahoo.com, smitasingh02@gmail.com
Objective:
To acquire work experience in the field of biotechnology.
Education : Year
M.S, Biotechnology December 13th, 2008
Florida Institute of Technology
Melbourne, FL
GPA 3.36/4.00
Relevant Courses:
Molecular Genetics
DNA Structure and Function
Signal Transduction
Biomaterials
Plant Physiology
Master of Science, Biotechnology
Pravara Rural University, Loni, India July, 2007
GPA 2.95/4
Relevant Course
Cell Biology
Pharmaceutical Biotechnology
Molecular Biology
Bioinformatics
Human Biochemistry
Genetic Engineering
Animal Tissue Culture
Immunology
Human Physiology
Introduction to Biostaistics
Medical Microbiology
Regulation and Patenting
Work Experience:
Internship – Florida Institute Technology.
During my internship at Florida Institute of Technology I was responsible for a project which examined the concentration dependence of a hormone, 1-MA on starfish oocyte (egg) maturation. This project monitored the phosphorylation of MAP Kinase and MEK, which are specific protein kinases that function in a cascade controlling cell growth and differentiation. The phosphorylation of these proteins occurs on addition of a hormone, 1-Methyladinine. This hormone was added at different concentrations and different time intervals to determine a threshold level that induces oocyte maturation. As a part of this project, I learned techniques of cell and molecular biology, including Western Blotting and Microinjection. The internship provided the opportunity to do independent basic bench research under the mentorship of Dr. David Carroll (Letters of Recommendation available upon request). At the end of the internship and as part of the degree requirement a departmental oral powerpoint presentation was given.
Internship – Pravara Rural University, Loni, India.
As a part of my Post Graduate studies, I worked on a project entitled “Anti Cancer Activity of a Compound, Camptothecin”. Camptothecin is a plant derivative. This project included the screening of different parts of a plant called Camptotheca nimmoniana for an anticancer bioactive compound called camptothecin. The plant parts that were tested were the leaves stem and seedlings. A comparative study on the amount of camptothecin produced by each plant part was developed. This compound was bioassayed using a cancerous cell line and standard Tissue Culture Techniques.
Internship at Haffkins Institute for Training Research & Testing, India
Course: “ On the Job Training “ conducted by the Department of Clinical Pathology, Virology & Zoonoses.
We worked on various tests or bioassays to diagnose certain virus-related diseases. Examples of the tests performed are the ELISA and Western Blotting, both used for protein and antibody determinations. We also did PCR analysis of certain genes involved in a disease called tuberculosis.
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Internship at Padmashree Institute of Information Sciences, Bangalore, India.
Course: Diploma in Plant Tissue Culture & Secondary Metabolite Production.
During this internship I trained to learn various plant tissue culture techniques. We also practiced developing Callus cultures and the techniques in the stages of culturing genetically engineered plants.
Seminars
I attended a seminar conducted by Prof. V.C. Vivekanandan which was on Patents Law. He
talked about the Patents Law and described the global perspective of it.
Assignments
As a part of my assignment I researched antigen-specific T-cell immunity. At the completion of this research, I presented a paper published on 9th March 2005 by Proceeding of National Academy of Sciences (PNAS) of USA. The authors Lili Yang and David Baltimore mentioned about the long term in – vivo provision of antigen-specific T-cell immunity by programming hematopoietic stem cells.
Reference: Long-term in vivo provision of antigen-specific T cell immunity by programming hematopoietic stem cells
Lili Yang and David Baltimore
PNAS, Mar 2005; 102: 4518 – 4523
Technical Skills:
Microsoft Word, Microsoft Powerpoint, Microsoft office Excel.
Languages spoken:
English, Hindi (Indian).
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